DetectEV: A functional enzymatic assay to assess integrity and bioactivity of extracellular vesicles.

ABSTRACT: The application of extracellular vesicles (EVs) as therapeutics or nanocarriers in cell-free therapies necessitates meticulous evaluations of different features, including their identity, bioactivity, batch-to-batch reproducibility, and stability. Given the inherent heterogeneity in EV preparations, this assessment demands sensitive functional assays to provide key quality control metrics, complementing established methods to ensure that EV preparations meet the required functionality and quality standards. Here, we introduce the detectEV assay, an enzymatic-based approach for assessing EV luminal cargo bioactivity and membrane integrity. This method is fast, cost-effective, and quantifiable through enzymatic units. Utilizing microalgae-derived EVs, known as nanoalgosomes, as model systems

SUBMITTER: Adamo G 

PROVIDER: S-EPMC11705427 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature