HnRNPU-mediated pathogenic alternative splicing drives gastric cancer progression.

ABSTRACT:

Background

Alternative splicing (AS) is a process that facilitates the differential inclusion of exonic sequences from precursor messenger RNAs, significantly enhancing the diversity of the transcriptome and proteome. In cancer, pathogenic AS events are closely related to cancer progression. This study aims to investigate the role and regulatory mechanisms of AS in gastric cancer (GC).

Methods

We analyzed AS events in various tumor samples and identified hnRNPU as a key splicing factor in GC. The effects of hnRNPU on cancer progression were assessed through in vitro and in vivo experiments. Gene knockout models and the FTO inhibitor (meclofenamic acid) were used to validate the interaction between hnRNPU and FTO and their impact on AS.

Results

We found that hnRNPU ser

SUBMITTER: Jin G 

PROVIDER: S-EPMC11705778 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature