New multilocus sequence typing scheme for Enterococcus faecium reveals sequential outbreaks of vancomycin-resistant E. faecium ST1162 and ST610 in a Japanese tertiary medical center.

ABSTRACT: Vancomycin-resistant Enterococcus faecium (VREfm) is a major nosocomial pathogen, and molecular epidemiological tools are crucial for controlling its spread. Pulsed-field gel electrophoresis (PFGE) is still used in clinical laboratories despite the increased accessibility of whole-genome sequencing (WGS). As PFGE equipment is no longer commercially available, clinical laboratories need alternative tools. Highly standardized multilocus sequence typing (MLST) is one option. However, the original MLST scheme for E. faecium, designed in 2002, showed inconsistencies with WGS-based typing. Therefore, the new Bezdíček MLST scheme, which offers more accurate genetic similarity based on genome-wide data, has recently been proposed. To clarify its clinical utility in analyzing nosocomi

SUBMITTER: Karino M 

PROVIDER: S-EPMC11705941 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature