Comparative analysis of improved m6A sequencing based on antibody optimization for low-input samples.

ABSTRACT: The most effective method for mapping N6-methyladenosine (m⁶A) is m⁶A RNA immunoprecipitation sequencing (MeRIP-seq). The quality of MeRIP-seq relies on various factors, with the anti-m⁶A antibody being a crucial determinant. However, comprehensive research on anti-m⁶A antibody selection and optimal concentrations for different tissues has been limited. In this study, we optimized the concentration of five different anti-m⁶A antibodies across various tissues. Our findings demonstrated that 5 µg of Millipore antibodies (ABE572 and MABE1006) performed well, starting from 15 µg total RNA from the liver, while 1.25 µg of Cell Signaling Technology antibodies (CST) (#56593) was suitable for low-input total RNA. In summary, we provide a signi

SUBMITTER: Lu J 

PROVIDER: S-EPMC11707076 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature