De novo whole-genome assembly and annotation of Coffea arabica var. Geisha, a high-quality coffee variety from the primary origin of coffee.

ABSTRACT: Geisha coffee is recognized for its unique aromas and flavors and, accordingly, has achieved the highest prices in the specialty coffee markets. We report the development of a chromosome-level, well-annotated, genome assembly of Coffea arabica var. Geisha. Geisha is considered an Ethiopian landrace that represents germplasm from the Ethiopian center of origin of coffee. We used a hybrid de novo assembly approach combining 2 long-read single molecule sequencing technologies, Oxford Nanopore and Pacific Biosciences, together with scaffolding with Hi-C libraries. The final assembly is 1.03 Gb in size with BUSCO assessment of the assembly completeness of 97.7% of single-copy orthologs clusters. RNA-Seq and Iso-Seq data were used as transcriptional experimental evidence for annotation and gene

SUBMITTER: Medrano JF 

PROVIDER: S-EPMC11708220 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature