Elucidating the role of carrier proteins in cytokine stabilization within double emulsion-based polymeric nanoparticles.

ABSTRACT: Polymeric micro- and nanoparticles are useful vehicles for delivering cytokines to diseased tissues such as solid tumors. Double emulsion solvent evaporation is one of the most common techniques to formulate cytokines into vehicles made from hydrophobic polymers; however, the liquid-liquid interfaces formed during emulsification can greatly affect the stability and therapeutic performance of encapsulated cytokines. To develop more effective cytokine-delivery systems, a clear molecular understanding of the interactions between relevant proteins and solvents used in the preparation of such particles is needed. We utilized an integrated computational and experimental approach for studying the governing mechanisms by which interleukin-12 (IL-12), a clinically relevant cytokine, is protected fr

SUBMITTER: Rhodes ER 

PROVIDER: S-EPMC11711224 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature