Insights into Treponema pallidum genomics from modern and ancient genomes using a novel mapping strategy.

ABSTRACT:

Background

Treponemal diseases are a significant global health risk, presenting challenges to public health and severe consequences to individuals if left untreated. Despite numerous genomic studies on Treponema pallidum and the known possible biases introduced by the choice of the reference genome used for mapping, few investigations have addressed how these biases affect phylogenetic and evolutionary analysis of these bacteria. In this study, we ascertain the importance of selecting an appropriate genomic reference on phylogenetic and evolutionary analyses of T. pallidum.

Results

We designed a multiple-reference-based (MRB) mapping strategy using four different reference genomes and compared it to traditional single-reference mapping. To conduct this comparison, we created

SUBMITTER: Pla-Diaz M 

PROVIDER: S-EPMC11716147 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature