Metabolic Atlas of Human Eyelid Infiltrative Basal Cell Carcinoma.

ABSTRACT:

Purpose

Eyelid infiltrative basal cell carcinoma (iBCC) is the most common malignant tumor affecting the ocular adnexa, but studies on metabolic changes within its microenvironment and heterogeneity at the tumor invasive area are limited. This study aims to analyze metabolic differences among iBCC cell types using single-cell and spatial metabolomics analysis and to examine metabolic environment at the tumor invasive area.

Methods

Single-cell transcriptomic data of human basal cell carcinoma (BCC) were clustered and visualized using Uniform Manifold Approximation and Projection. Metabolic reprogramming was analyzed with single-cell flux estimation analysis. Spatial metabolomics data were obtained with the Timstof Flex MALDI 2 system, and Bruker software was used for region s

SUBMITTER: Huang Y 

PROVIDER: S-EPMC11717128 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature