A high-throughput platform for single-molecule tracking identifies drug interaction and cellular mechanisms.

ABSTRACT: The regulation of cell physiology depends largely upon interactions of functionally distinct proteins and cellular components. These interactions may be transient or long-lived, but often affect protein motion. Measurement of protein dynamics within a cellular environment, particularly while perturbing protein function with small molecules, may enable dissection of key interactions and facilitate drug discovery; however, current approaches are limited by throughput with respect to data acquisition and analysis. As a result, studies using super-resolution imaging are typically drawing conclusions from tens of cells and a few experimental conditions tested. We addressed these limitations by developing a high-throughput single-molecule tracking (htSMT) platform for pharmacologic dissection of

SUBMITTER: McSwiggen DT 

PROVIDER: S-EPMC11717362 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature