Dominant genetic screen for cofactors that enhance antisense RNA-mediated gene silencing in fission yeast.

ABSTRACT: Specific gene silencing has been demonstrated in a number of organisms by the introduction of antisense RNA. Mutagenesis of host-encoded factors has begun to unravel the mechanism of several forms of RNA-mediated gene silencing and has suggested that it may have been conserved through evolution. This has led to the identification of certain host genes, which, when mutated, abrogate this phenomenon. Conversely, the identification of other factors that, when co-expressed or overexpressed, can enhance gene inhibition is equally important for both elucidating the mechanism of this process and enhancing gene silencing in recalcitrant systems. We have taken such a dominant genetic approach to identify several host-encoded factors that dramatically enhance target gene silencing when co-expressed

SUBMITTER: Raponi M 

PROVIDER: S-EPMC117174 | biostudies-literature | 2002 Jun

REPOSITORIES: biostudies-literature