Development of a new flippase-dependent mouse model for red fluorescence-based isolation of KRAS^G12D oncogene-expressing tumor cells.

ABSTRACT: Proto-oncogene KRAS, GTPase (KRAS) is one of the most intensively studied oncogenes in cancer research. Although several mouse models allow for regulated expression of mutant KRAS, selective isolation and analysis of transforming or tumor cells that produce the KRAS oncogene remains a challenge. In our study, we present a knock-in model of oncogenic variant KRAS^G12D that enables the "activation" of KRAS^G12D expression together with production of red fluorescent protein tdTomato. Both proteins are expressed from the endogenous Kras locus after recombination of a transcriptional stop box in the genomic DNA by the enzyme flippase (Flp). We have demonstrated the functionality of the allele termed RedRas (abbreviated Kras^RR) under in vitro conditions with mouse

SUBMITTER: Hrckulak D 

PROVIDER: S-EPMC11717838 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature