Evaluating the impact of media and feed combinations on CHO cell culture performance and monoclonal antibody (trastuzumab) production.

ABSTRACT: The choice of media and feeds significantly influences the performance of Chinese Hamster Ovary (CHO) mammalian cell cultures in producing desired biologics like monoclonal antibodies (mAb). Sub-optimal nutrient feed/media composition can severely impact cell proliferation and the quality of the final mAb product. For instance, proper protein glycosylation, crucial for mAb stability, safety, and efficacy, heavily relies on cell culture conditions. Currently, starter CHO culture media and daily supplemental feeds used in industrial manufacturing consist of proprietary composition of nutrients critical for mAb production. Standardized optimal media/feed combinations necessary for different cell lines are often lacking, necessitating individualized optimization for each cell line and mAb prod

SUBMITTER: Gyorgypal A 

PROVIDER: S-EPMC11718031 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature