Highly effective batch effect correction method for RNA-seq count data.

ABSTRACT: RNA sequencing (RNA-seq) has become a cornerstone of transcriptomics, providing detailed insights into gene expression across diverse biological conditions and sample types. However, RNA-seq data are often confounded by batch effects, systematic non-biological variations that compromise data reliability and obscure true biological differences. To address these challenges, we introduce ComBat-ref, a refined batch effect correction method designed to enhance the statistical power and reliability of differential expression analysis in RNA-seq data. Building on the principles of ComBat-seq, ComBat-ref employs a negative binomial model for count data adjustment but innovates by selecting a reference batch with the smallest dispersion, preserving count data for the reference batch, and adjusting

SUBMITTER: Zhang X 

PROVIDER: S-EPMC11718288 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature