A Two-Step Protocol for Isolation and Maintenance of Lung Cancer Primary 3D Cultures.

ABSTRACT:

Background/objectives

Despite the introduction of innovative therapeutics, lung cancer is still the leading cause of cancer-related death. For this reason, lung cancer still requires deep characterization to identify cellular and molecular targets that can be used to develop novel therapeutic strategies. Three-dimensional cellular models, including patient-derived organoids (PDOs), represent useful tools to study lung cancer biology and may be employed in the future as predictive tools in therapeutic decisions. However, the successful establishment of lung cancer organoids cultures that faithfully represent the respective patient tissues is still challenging due to low success rate and/or overgrowth of normal airway epithelial cells.

Methods

We set up a two-step protocol tha

SUBMITTER: Strocchi S 

PROVIDER: S-EPMC11718983 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature