Prolonged Hypoxia in Rat Living Myocardial Slices Affects Function, Expression, and Structure.

ABSTRACT: Ischemic heart disease is the leading cause of death worldwide. Reduced oxygen supply and myocardial hypoxia lead to tissue damage and impairment of the heart function. To the best of our knowledge, the primary functional effects of hypoxia in the multicellular model of living myocardial slices (LMSs) have not been investigated so far. In this study, we analyzed force generation, ultrastructure, gene expression, and proteome changes in rat LMS after 24 h of ex vivo culture in normal and reduced levels of oxygen (O₂). We observed a significant reduction in absolute force and a slowdown of force kinetics as well as an increase in cardiomyocyte apoptosis and myofibrillar and mitochondrial damage, as well as transcriptomic changes. Proteome analysis revealed the deregulation of prot

SUBMITTER: Waleczek FJG 

PROVIDER: S-EPMC11720517 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature