Characterisation of a Japanese Encephalitis virus genotype 4 isolate from the 2022 Australian outbreak.

ABSTRACT: Human infections with the Japanese encephalitis virus (JEV) are a leading cause of viral encephalitis. An unprecedented outbreak of JEV genotype 4 was recently reported in Australia, with an isolate (JEV_NSW/22) obtained from a stillborn piglet brain. Herein we conduct a thorough characterization of JEV_NSW/22 in three different mouse strains and in human cortical brain organoids (hBOs), and determined the ability of JEV_NSW/22 to be neutralized by sera from humans vaccinated with IMOJEV. JEV_NSW/22 was less virulent than JEV_FU (genotype 2) and JEV_Nakayama (genotype 3) in C57BL/6J mice and in interferon regulatory factor 7 deficient (Irf7^-/-) mice, with infection of wild-type and knockout murine embryonic fibroblasts

SUBMITTER: Nguyen W 

PROVIDER: S-EPMC11721158 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature