Identification of biomarkers for chronic renal fibrosis and their relationship with immune infiltration and cell death.

ABSTRACT:

Background

Chronic kidney disease (CKD) represents a significant global public health challenge. This study aims to identify biomarkers of renal fibrosis and elucidate the relationship between unilateral ureteral obstruction (UUO), immune infiltration, and cell death.

Methods

Gene expression matrices for UUO were retrieved from the gene expression omnibus (GSE36496, GSE79443, GSE217650, and GSE217654). Seven genes identified through Protein-Protein Interaction (PPI) network and Support Vector Machine-Recursive Feature Elimination (SVM-RFE) analysis were validated using qRT-PCR in both in vivo and in vitro UUO experiments. WB assays were employed to investigate the role of Clec4n within NF-κB signaling pathway in renal fibrosis. The composition of immune cells i

SUBMITTER: Shi J 

PROVIDER: S-EPMC11721624 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature