Exploring Evolution to Uncover Insights Into Protein Mutational Stability.

ABSTRACT: Determining the impact of mutations on the thermodynamic stability of proteins is essential for a wide range of applications such as rational protein design and genetic variant interpretation. Since protein stability is a major driver of evolution, evolutionary data are often used to guide stability predictions. Many state-of-the-art stability predictors extract evolutionary information from multiple sequence alignments of proteins homologous to a query protein, and leverage it to predict the effects of mutations on protein stability. To evaluate the power and the limitations of such methods, we used the massive amount of stability data recently obtained by deep mutational scanning to study how best to construct multiple sequence alignments and optimally extract evolutionary information fr

SUBMITTER: Hermans P 

PROVIDER: S-EPMC11721782 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature