Polyamide Microparticles with Immobilized Enological Pectinase as Efficient Biocatalysts for Wine Clarification: The Role of the Polymer Support.

ABSTRACT: Free pectinase is commonly employed as a biocatalyst in wine clarification; however, its removal, recovery, and reuse are not feasible. To address these limitations, this study focuses on the immobilization of a commercial pectinolytic preparation (Pec) onto highly porous polymer microparticles (MPs). Seven microparticulate polyamide (PA) supports, namely PA4, PA6, PA12 (with and without magnetic properties), and the copolymeric PA612 MP, were synthesized through activated anionic ring-opening polymerization of various lactams. Pectinase was non-covalently immobilized on these supports by adsorption, forming Pec@PA conjugates. Comparative activity and kinetic studies revealed that the Pec@PA12 conjugate exhibited more than twice the catalytic efficiency of the free enzyme, followed by Pec@

SUBMITTER: Oliveira SC 

PROVIDER: S-EPMC11722140 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature