Widespread 3D genome reorganization precedes programmed DNA rearrangement in <i>Oxytricha trifallax</i>.

ABSTRACT: Genome organization recapitulates function, yet ciliates like Oxytricha trifallax possess highly-specialized germline genomes, which are largely transcriptionally silent. During post-zygotic development, Oxytricha's germline undergoes large-scale genome editing, rearranging precursor genome elements into a transcriptionally-active genome with thousands of gene-sized nanochromosomes. Transgenerationally-inherited RNAs, derived from the parental somatic genome, program the retention and reordering of germline fragments. Retained and eliminated DNA must be distinguished and processed separately, but the role of chromatin organization in this process is unknown. We developed tools for studying Oxytricha nuclei and apply them to map the 3D organization of precursor and deve

SUBMITTER: Villano DJ 

PROVIDER: S-EPMC11722245 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature