DNA supercoiling modulates eukaryotic transcription in a gene-orientation dependent manner.

ABSTRACT: Transcription introduces torsional stress in the DNA fiber causing it to transition from a relaxed to a supercoiled state that can propagate across several kilobases and modulate the binding and activity of DNA-associated proteins. As a result, transcription at one locus has the potential to impact nearby transcription events. In this study, we asked how DNA supercoiling affects histone modifications and transcription of neighboring genes in the multicellular eukaryote Caenorhabditis elegans. We acutely depleted the two major topoisomerases and measured nascent transcription by Global Run-on sequencing (GRO-seq), RNA Polymerase II occupancy by ChIP-seq, gene expression by RNA-seq and four transcription-associated histone modifications by Cut & Tag. Depletion of topoisomerases I and

SUBMITTER: Morao AK 

PROVIDER: S-EPMC11722375 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature