Single-cell mapping of regulatory DNA:Protein interactions.

ABSTRACT: Gene expression is coordinated by a multitude of transcription factors (TFs), whose binding to the genome is directed through multiple interconnected epigenetic signals, including chromatin accessibility and histone modifications. These complex networks have been shown to be disrupted during aging, disease, and cancer. However, profiling these networks across diverse cell types and states has been limited due to the technical constraints of existing methods for mapping DNA:Protein interactions in single cells. As a result, a critical gap remains in understanding where TFs or other chromatin remodelers bind to DNA and how these interactions are perturbed in pathological contexts. To address this challenge, we developed a transformative single-cell immuno-tethering DNA:Protein mapping techno

SUBMITTER: Chi WY 

PROVIDER: S-EPMC11722406 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature