Single-nucleus transcriptomics reveals time-dependent and cell-type-specific effects of psilocybin on gene expression.

ABSTRACT: There is growing interest to investigate classic psychedelics as potential therapeutics for mental illnesses. Previous studies have demonstrated that one dose of psilocybin leads to persisting neural and behavioral changes. The durability of psilocybin's effects suggests that there are likely alterations of gene expression at the transcriptional level. In this study, we performed single-nucleus RNA sequencing of the dorsal medial frontal cortex of male and female mice. Samples were collected at 1, 2, 4, 24, or 72 hours after psilocybin or ketamine administration and from control animals. At baseline, major excitatory and GABAergic cell types selectively express particular serotonin receptor transcripts. The psilocybin-evoked differentially expressed genes in excitatory neurons were involve

SUBMITTER: Liao C 

PROVIDER: S-EPMC11722411 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature