Development of a Novel Amplifiable System to Quantify Hydrogen Peroxide in Living Cells.

ABSTRACT: Although many redox signaling molecules are present at low concentrations, typically ranging from micromolar to submicromolar levels, they often play essential roles in a wide range of biological pathways and disease mechanisms. However, accurately measuring low-abundant analytes has been a significant challenge due to the lack of sensitivity and quantitative capability of existing measurement methods. In this study, we introduced a novel chemically induced amplifiable system for quantifying low-abundance redox signaling molecules in living cells. We utilized H₂O₂ as a proof-of-concept analyte and developed a probe that quantifies cellular peroxide levels by combining the NanoBiT system with androgen receptor dimerization as a reporting mechanism. Our system demonstra

SUBMITTER: Wang L 

PROVIDER: S-EPMC11722959 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature