Identification of U6 Promoter and Establishment of Gene-Editing System in Larix kaempferi (Lamb.) Carr.

ABSTRACT: This study aimed to establish a CRISPR/Cas9 gene-editing system for Larix kaempferi (Lamb.) Carr. (Japanese larch). We screened L. kaempferi U6 promoters and used them to drive sgRNA expression in the CRISPR/Cas9 gene-editing system. The L. kaempferi embryogenic callus was used as the receptor material for genetic transformation, and the frequency and types of gene editing were then analyzed. The results showed various mutations in the transgenic materials, including base substitutions and deletions, and the editing frequency ranged from 5% to 14.29%. In summary, we established a CRISPR/Cas9 gene-editing system for L. kaempferi. Our results demonstrate that the CRISPR/Cas9 system can efficiently edit genes in L. kaempferi, with significantly higher editin

SUBMITTER: Xing JX 

PROVIDER: S-EPMC11722980 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature