An Improved P(V) Thio-Oligonucleotide Synthesis Platform.

ABSTRACT: Three critical advances in simplifying the adoption of P(V)-based stereopure, phosphorothioate-containing oligonucleotide synthesis are reported. A more inexpensive phosphorus-sulfur incorporation reagent (Ψ^Br) is introduced, a robust linker system was developed, and a systematic study of common nucleobase protecting groups was performed to significantly reduce the barrier to adoption of this technology.

SUBMITTER: Nassir M 

PROVIDER: S-EPMC11723805 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature