Integrated proteomics and scRNA-seq analyses of ovarian cancer reveal molecular subtype-associated cell landscapes and immunotherapy targets.

ABSTRACT:

Background

Epithelial ovarian cancer (EOC) represents the most lethal gynaecological malignancy, yet understanding the connections between its molecular subtypes and their therapeutic implications remains incomplete.

Methods

We conducted mass spectrometry-based proteomics analyses of 154 EOC tumour samples and 29 normal fallopian tubes, and single-cell RNA sequencing (scRNA-seq) analyses of an additional eight EOC tumours to classify proteomic subtypes and assess their cellular ecosystems and clinical significance. The efficacy of identified therapeutic targets was evaluated in patient-derived xenograft (PDX) and orthotopic mouse models.

Results

We identified four proteomic subtypes with distinct clinical relevance: malignant proliferative (C1), immune infiltrating (C

SUBMITTER: Tan R 

PROVIDER: S-EPMC11723995 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature