Impedance mapping with high-density microelectrode array chips reveals dynamic heterogeneity of in vitro epithelial barriers.

ABSTRACT: Epithelial tissues in vitro undergo dynamic changes while differentiating heterogeneously on the culture substrate. This gives rise to diverse cellular arrangements which are undistinguished by conventional analysis approaches, such as transepithelial electrical resistance measurement or permeability assays. In this context, solid substrate-based systems with integrated electrodes and electrochemical impedance monitoring capability can address the limited spatiotemporal resolution of traditional porous membrane-based methods. This label-free technique facilitates local, continuous, long-term analysis of tissue barrier properties for organ-on-chip applications. Increasing spatial resolution requires small electrodes arranged in a dense array, known as high-density microelectrode arrays (HD-

SUBMITTER: Venz A 

PROVIDER: S-EPMC11724121 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature