Extracellular vesicles of <i>Lactiplantibacillus plantarum</i> PCM 2675 and <i>Lacticaseibacillus rhamnosus</i> PCM 489: an introductory characteristic.

ABSTRACT: Aim: Extracellular vesicles (EVs) are involved in intercellular and interkingdom communication in the complex communities that constitute the niche-specific microbiome of the colonized host. Therefore, studying the structure and content of EVs produced by resident bacteria is crucial to understanding their functionality and impact on the host and other microorganisms. Methods: Bacterial EVs were isolated by differential centrifugation, their size and concentration were measured by transmission electron microscopy and nanoparticle tracking analysis, and the cargo proteins were identified by liquid chromatography coupled to tandem mass spectrometry. The cytotoxicity of bacterial EVs was tested using the human epithelial cell line A549 and an in vivo model of Galleria

SUBMITTER: Kowalik K 

PROVIDER: S-EPMC11725429 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature