Characterization of intact FeoB in a lipid bilayer using styrene-maleic acid (SMA) copolymers.

ABSTRACT: The acquisition of ferrous iron (Fe²⁺) is crucial for the survival of many pathogenic bacteria living within acidic and/or anoxic conditions such as Vibrio cholerae, the causative agent of the disease cholera. Bacterial pathogens utilize iron as a cofactor to drive essential metabolic processes, and the primary prokaryotic Fe²⁺ acquisition mechanism is the ferrous iron transport (Feo) system. In V. cholerae, the Feo system comprises two cytosolic proteins (FeoA, FeoC) and a complex, polytopic transmembrane protein (FeoB) that is regulated by an N-terminal soluble domain (NFeoB) with promiscuous NTPase activity. While the soluble components of the Feo system have been frequently studied, very few reports exist on the intact membrane protein FeoB. Moreover, FeoB has bee

SUBMITTER: Lee M 

PROVIDER: S-EPMC11725443 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature