A comprehensive human embryo reference tool using single-cell RNA-sequencing data.

ABSTRACT: Stem cell-based embryo models offer unprecedented experimental tools for studying early human development. The usefulness of embryo models hinges on their molecular, cellular and structural fidelities to their in vivo counterparts. To authenticate human embryo models, single-cell RNA sequencing has been utilized for unbiased transcriptional profiling. However, an organized and integrated human single-cell RNA-sequencing dataset, serving as a universal reference for benchmarking human embryo models, remains unavailable. Here we developed such a reference through the integration of six published human datasets covering development from the zygote to the gastrula. Lineage annotations are contrasted and validated with available human and nonhuman primate datasets. Using stabilized Uniform Mani

SUBMITTER: Zhao C 

PROVIDER: S-EPMC11725501 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature