Complex transcription regulation of acidic chitinase suggests fine-tuning of digestive processes in Drosera binata.

ABSTRACT:

Main conclusion

DbChitI-3, Drosera binata's acidic chitinase, peaks at pH 2.5 from 15 °C to 30 °C. Gene expression is stimulated by polysaccharides and suppressed by monosaccharide digestion, implying a feedback loop in its transcriptional regulation. Here, we characterised a novel chitinase gene (DbChitI-3) isolated from the carnivorous plant species Drosera binata with strong homology to other Drosera species' extracellular class I chitinases with a role in digestive processes. The capability to cleave different forms of chitin was tested using recombinantly produced chitinase in Escherichia coli (rDbChitI-3^S-His) and subsequent purification. The recombinant protein did not cleave chitin powder, the mono-, di- and tri- N-acetyl-D-glucosamine substrates, but cleaved ace

SUBMITTER: Mikitova V 

PROVIDER: S-EPMC11725546 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature