Discovering genetic modulators of the protein homeostasis system through multilevel analysis.

ABSTRACT: Every protein progresses through a natural lifecycle from birth to maturation to death; this process is coordinated by the protein homeostasis system. Environmental or physiological conditions trigger pathways that maintain the homeostasis of the proteome. An open question is how these pathways are modulated to respond to the many stresses that an organism encounters during its lifetime. To address this question, we tested how the fitness landscape changes in response to environmental and genetic perturbations using directed and massively parallel transposon mutagenesis in Caulobacter crescentus. We developed a general computational pipeline for the analysis of gene-by-environment interactions in transposon mutagenesis experiments. This pipeline uses a combination of general linear

SUBMITTER: Sarsani V 

PROVIDER: S-EPMC11725641 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature