Binding mode-guided development of high-performance antibodies targeting site-specific posttranslational modifications.

ABSTRACT: Posttranslational modifications (PTMs) of proteins play critical roles in regulating many cellular events. Antibodies targeting site-specific PTMs are essential tools for detecting and enriching PTMs at sites of interest. However, fundamental difficulties in molecular recognition of both PTM and surrounding peptide sequence have hindered the efficient generation of highly sequence-specific anti-PTM antibodies. Furthermore, the widespread use of potentially inconsistent, nonrenewable, and molecularly undefined antibodies presents experimental challenges thought to contribute to the reproducibility problem in biomedical research. In this study, we describe the binding mode-guided development of a platform that efficiently generates potent and selective recombinant antibodies to PTMs that are

SUBMITTER: Riso M 

PROVIDER: S-EPMC11725865 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature