Structural insight into sodium ion pathway in the bacterial flagellar stator from marine <i>Vibrio</i>.

ABSTRACT: Many bacteria swim in liquid or swarm on surface using the flagellum rotated by a motor driven by specific ion flow. The motor consists of the rotor and stator, and the stator converts the energy of ion flow to mechanical rotation. However, the ion pathway and the mechanism of stator rotation coupled with specific ion flow are still obscure. Here, we determined the structures of the sodium-driven stator of Vibrio, namely PomAB, in the presence and absence of sodium ions and the structure with its specific inhibitor, phenamil, by cryo-electron microscopy. The structures and following functional analysis revealed the sodium ion pathway, the mechanism of ion selectivity, and the inhibition mechanism by phenamil. We propose a model of sodium ion flow coupled with the stator rotation bas

SUBMITTER: Nishikino T 

PROVIDER: S-EPMC11725901 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature