Integrin-activating <i>Yersinia</i> protein Invasin sustains long-term expansion of primary epithelial cells as 2D organoid sheets.

ABSTRACT: Matrigel^®/BME^®, a basement membrane-like preparation, supports long-term growth of epithelial 3D organoids from adult stem cells [T. Sato et al., Nature 459, 262-265 (2009); T. Sato et al., Gastroenterology 141, 1762-1772 (2011)]. Here, we show that interaction between Matrigel's major component laminin-111 with epithelial α6β1-integrin is crucial for this process. The outer membrane protein Invasin of Yersinia is known to activate multiple integrin-β1 complexes, including integrin α6β1. A C-terminal integrin-binding fragment of Invasin, coated on culture plates, mediated gut epithelial cell adhesion. Addition of organoid growth factors allowed multipassage expansion in 2D. Polarization, junction formation, and generati

SUBMITTER: Wijnakker JJAPM 

PROVIDER: S-EPMC11725944 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature