Disparate Pathways for Extrachromosomal DNA Biogenesis and Genomic DNA Repair.

ABSTRACT:

Significance

Our study harnesses a CRISPR-based method to examine ecDNA biogenesis, uncovering efficient circularization between double-strand breaks. ecDNAs and their corresponding chromosomal scars can form via nonhomologous end joining or microhomology-mediated end joining, but the ecDNA and scar formation processes are distinct. Based on our findings, we establish a mechanistic model of excisional ecDNA formation.

SUBMITTER: Rose JC 

PROVIDER: S-EPMC11726015 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature