Toll-like receptor agonists promote the formation of tertiary lymphoid structure and improve anti-glioma immunity.

ABSTRACT:

Background

Glioma, characterized by limited lymphocytic infiltration, constitutes an "immune-desert" tumor displaying insensitivity to various immunotherapies. This study aims to explore therapeutic strategies for inducing tertiary lymphoid structure (TLS) formation within the glioma microenvironment (GME) to transition it from an immune resistant to an activated state.

Methods

TLS formation in GME was successfully induced by intracranial administration of Toll-like receptor (TLR) agonists (OK-432, TLR2/4/9 agonist) and glioma antigens (i.c. αTLR-mix). We employed staining analysis, antibody neutralization, single-cell RNA sequencing (scRNA-Seq), and BCR/TCR sequencing to investigate the underlying mechanisms of TLS formation and its role in anti-glioma immunity. Additionall

SUBMITTER: Shen S 

PROVIDER: S-EPMC11726345 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature