Elucidating the Mechanism of Radiation Therapy on Mesenchymal Cell Fate in Preventing Heterotopic Ossification.

ABSTRACT:

Objective

To recapitulate the use of radiation in preventing heterotopic ossification (HO) in an animal model to thereby mechanistically investigate radiation-induced changes at the single-cell level.

Background

HO is the formation of extra-skeletal bone in abnormal areas including muscle and soft tissue. Radiation therapy is a clinically proven, localized preventive measure for HO. Despite its efficacy, there is a lack of standardization of radiation prescription; however, the mechanism of the impact of radiation on HO prevention remains unknown.

Methods

C57BL6J male mice underwent burn/tenotomy with and without perioperative radiation treatment. Single-cell RNA sequencing was performed to analyze downstream signaling after HO-forming injury. Immunofluorescence micro

SUBMITTER: Hsieh ML 

PROVIDER: S-EPMC11726538 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature