RELT Is Upregulated in Breast Cancer and Induces Death in Breast Cancer Cells.

ABSTRACT:

Background

Receptor Expressed in Lymphoid Tissues (RELT) is a TNFRSF member that has two paralogs, RELL1 and RELL2; the three proteins are collectively referred to as RELT family members (RELTfms).

Methods

We sought to evaluate RELT expression in cancerous cells by using real-time PCR, western blotting, flow cytometry, and immunohistochemistry (IHC). The mechanism of RELT-induced cell death was assessed by western blotting, flow cytometry, luciferase assays, and morphology staining. RELT localization was detected through immunofluorescence and western blotting, and co-immunoprecipitation was used to test whether a mutated RELT interacts with the OXSR1 kinase.

Results

RELT and RELL1 protein expression was significantly elevated in cell lines representing breast and lun

SUBMITTER: Batiste M 

PROVIDER: S-EPMC11727564 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature