Optimization of central carbon metabolism by Warburg effect of human cancer cell improves triterpenes biosynthesis in yeast.

ABSTRACT: Optimizing central carbon metabolism (CCM) represents an attractive and challenging strategy to improve the biosynthesis of valuable chemicals due to the complex regulation of the CCM in yeast. In this study, we triggered the similar Warburg effect of cancer cells in yeast strains by introducing the human hypoxia-inducible factor-1 (HIF-1) complex, which regulated the expression of numerous enzymes involved in CCM and redirected the metabolic flux from glycolysis to tricarboxylic acid cycle. This redirection promoted the production of squalene to a 2.7-fold increase than the control strain BY4741. Furthermore, the HIF-1 complex boosted the production of represented endogenous triterpenoid ergosterol to 1145.95 mg/L, and exogenous triterpenoid lupeol to 236.35 mg/L in shake flask cultivatio

SUBMITTER: Lin X 

PROVIDER: S-EPMC11727583 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature