Uncovering the genetic basis of antiviral polyketide limocrocin biosynthesis through heterologous expression.

ABSTRACT:

Background

Streptomyces roseochromogenes NRRL 3504 produces clorobiocin, an aminocoumarin antibiotic that inhibits DNA replication. No other natural products have been isolated from this bacterium so far, despite the presence of a rich repertoire of specialized metabolite biosynthesis gene clusters (smBGCs) within its genome. Heterologous expression of smBGCs in suitable chassis speeds up the discovery of the natural products hidden behind these sets of genes.

Results

In this work we focus on one intriguing smBGC of NRRL 3504 bearing some similarity to gene clusters involved in production of manumycin family polyketides. Through heterologous expression in Streptomyces chassis strains S. albus Del14 and S. lividans ΔYA9, this smBGC (hereafter referred to as lim BGC) was shown

SUBMITTER: Melnyk S 

PROVIDER: S-EPMC11727611 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature