Divergent Contribution of Cytoplasmic Actins to Nuclear Structure of Lung Cancer Cells.

ABSTRACT: A growing body of evidence suggests that actin plays a role in nuclear architecture, genome organisation, and regulation. Our study of human lung adenocarcinoma cells demonstrates that the equilibrium between actin isoforms affects the composition of the nuclear lamina, which in turn influences nuclear stiffness and cellular behaviour. The downregulation of β-actin resulted in an increase in nuclear area, accompanied by a decrease in A-type lamins and an enhancement in lamin B2. In contrast, the suppression of γ-actin led to upregulation of the lamin A/B ratio through an increase in A-type lamins. Histone H3 post-translational modifications display distinct patterns in response to decreased actin isoform expression. The level of dimethylated H3K9me2 declined while acetylated H3K9ac increas

SUBMITTER: Shagieva G 

PROVIDER: S-EPMC11727787 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature