Innovative Methodology for Antimicrobial Susceptibility Determination in <i>Mycoplasma</i> Biofilms.

ABSTRACT: Mycoplasma spp. are facultative pathogens that contribute to the pathogenesis of multiple bovine diseases, including the bovine respiratory disease complex, and have been shown to form biofilms. Biofilm formation is associated with increased antibiotic resistance in many organisms, but accurate determination of antimicrobial susceptibility in biofilms is challenging. In Mycoplasma spp., antimicrobial susceptibility is routinely determined using metabolic pH-dependent color change. However, biofilm formation can lead to reduced metabolism, making interpretation of metabolic readouts difficult. Therefore, we developed and optimized a new flow cytometry-based method for antimicrobial susceptibility testing in biofilm-forming Mycoplasma, termed the live/dead antimicrobial

SUBMITTER: Jacobson BT 

PROVIDER: S-EPMC11728330 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature