Exploring the Venom Gland Transcriptome of <i>Bothrops asper</i> and <i>Bothrops jararaca</i>: De Novo Assembly and Analysis of Novel Toxic Proteins.

ABSTRACT: Previous proteomic studies of viperid venom revealed that it is mainly composed of metalloproteinases (SVMPs), serine proteinases (SVSPs), phospholipase A2 (PLA2), and C-type lectins (CTLs). However, other proteins appear in minor amounts that affect prey and need to be identified. This study aimed to identify novel toxic proteins in the venom gland transcriptome of Bothrops asper and Bothrops jararaca, using data from NCBI. Bioinformatics tools were used to assemble, identify, and compare potentially novel proteins in both species, and we performed functional annotation with BLASTX against the NR database. While previous assemblies have been performed for B. jararaca, this is the first assembly of the B. asper venom gland transcriptome. Proteins with potentiall

SUBMITTER: Espin-Angulo J 

PROVIDER: S-EPMC11728684 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature