Deciphering the nature and statistical optimization of antimicrobial metabolites of two endophytic bacilli.

ABSTRACT: In this study, Allium sativum, garlic, was selected to isolate endophytic bacteria and to evaluate the antimicrobial, antiviral, antioxidant, and cytotoxic activities of their produced metabolites followed by identification of the biosynthetic gene cluster of the antimicrobial metabolites using Oxford Nanopore Technology (ONT). Two bacterial isolates, C6 and C11, were found to have a broad-spectrum antagonistic effect against four standard microbial strains and were molecularly identified using 16 S ribosomal RNA sequence analysis and deposited in a local culture collection as B. velezensis CCASU-C6, and B. subtilis CCASU-C11, respectively. Optimization for the maximum production of antimicrobial metabolites revealed that a four-day incubation period was optimal, with sucrose and tryptone

SUBMITTER: Isleem RS 

PROVIDER: S-EPMC11730024 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature