Increased functional potency of multi-edited CAR-T cells manufactured by a non-viral transfection system.

ABSTRACT: Chimeric antigen receptor (CAR)-T cell therapy represents a breakthrough for the treatment of hematological malignancies. However, to treat solid tumors and certain hematologic cancers, next-generation CAR-T cells require further genetic modifications to overcome some of the current limitations. Improving manufacturing processes to preserve cell health and function of edited T cells is equally critical. Here, we report that Solupore, a Good Manufacturing Practice-aligned, non-viral physicochemical transfection system, can be used to manufacture multi-edited CAR-T cells using CRISPR-Cas9 ribonucleoproteins while maintaining robust cell functionality. When compared to electroporation, an industry standard, T cells that were triple edited using Solupore had reduced levels of apoptosis and mai

SUBMITTER: O'Sullivan A 

PROVIDER: S-EPMC11730244 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature