Fine tuning enzyme activity assays for monitoring the enzymatic hydrolysis of PET.

ABSTRACT: Efficient monitoring of the enzymatic PET-hydrolysis is crucial for developing novel plastic-degrading biocatalysts. Herein, we aimed to upgrade in terms of accuracy the analytical methods useful for monitoring enzymatic PET-degradation. For the HPLC-based assessment, the incorporation of an internal standard within the analytic procedure enabled a more accurate quantification of the overall TPA content and the assessment of molar distributions and relative content of each aromatic degradation product. The provided calibration curves cover a broad concentration range, from µM to low mM scale, facilitating assessment of both lower and higher PETase activities, with a limit of detection positioned below the reported PET-degrading activities. The increased reproducibility and accuracy of the

SUBMITTER: Boros K 

PROVIDER: S-EPMC11730307 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature