Uncovering the substrate of olefin synthase loading domains in cyanobacteria <i>Picosynechococcus</i> sp. strain PCC 7002.

ABSTRACT: Cyanobacteria are widespread, photosynthetic, gram-negative bacteria that generate numerous bioactive secondary metabolites via complex biosynthetic enzymatic machinery. The model cyanobacterium Picosynechococcus sp. strain PCC 7002, hereafter referred to as PCC 7002, contains a type I polyketide synthase (PKS), termed olefin synthase (OlsWT), that synthesizes 1-nonadecene and 1,14-nonadecadiene: α-olefins that are important for growth at low temperatures. The putative biochemistry encoded by the PKS domains suggests that OlsWT will create an olefin with one additional carbon relative to the original substrate (+1 mechanism). The first domain in the multi-module OlsWT protein has homology to fatty acyl-AMP ligases (FAALs) that typically activate free fatty acids prior to crea

SUBMITTER: Ynigez-Gutierrez AE 

PROVIDER: S-EPMC11730487 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature