Mustard Gas Induced Corneal Injury Involves Ferroptosis and p38 MAPK Signaling.

ABSTRACT:

Purpose

Sulfur mustard gas (SM) exposure to eyes causes multiple corneal injuries including stromal cell loss in vivo. However, mechanisms mediating stromal cell loss/death remains elusive. This study sought to test the novel hypothesis that SM-induced toxicity to human corneal stromal fibroblasts involves ferroptosis mechanism via p38 MAPK signaling.

Methods

New Zealand white rabbit corneas, naïve and SM exposed (200 mg-min/m3 for eight minutes and collected after three days) were used to examine the levels of cell death and reactive oxygen species (ROS) for in vivo studies. Donor human corneas were used to generate primary human corneal stromal fibroblasts (hCSF) for in vitro studies. The hCSFs were exposed to nitrogen mustard (NM; SM analogue) at various timepoints (30 mi

SUBMITTER: Sinha NR 

PROVIDER: S-EPMC11730948 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature